Enrichment of Glycopeptides
Protein glycosylation influence many biological processes such as protein-protein interaction, cell recognition, protein stability, etc. Changes in glycans composition are typicaly a consequence of different enzymes activities under non-physiological conditions. Altered glycans are good targets for biomarker discovery or for monitoring disease progression.
AffiPro has developed a new technology for detection of glycosylated peptides by funcionalized MALDI targets modified with different types of lectins. The enrichment strategy is simple, robust, time-and-money non-consuming. Two types of MALDI targets modified with Concanavalin A (ConA) or Wheat germ agglutinin (WGA) are now available for enrichment of high mannose or complex type glycans.