For detection and quantification of antigens from different sources, ELISA is the most often used method, mainly for its simplicity, sensitivity and precise quantification. The key role in ELISA plays the antibody, which determines the sensitivity and specificity of the assay. The major limitations of the ELISA technique are no information about biochemical properties of the antigen such as molecular weight and the possibility of less antibody specificity giving false positive results.
Functionalized MALDI targets developed by AffiPro enable detection of antigens by either mass spectrometry or chemiluminescence or by combination of both techniques. The benefit of this approach is the high specificity of mass spectrometric detection, which enables to determine the molecular weight of the antigen. AffiPro offers functionalized MALDI targets for detection of haptoglobin phenotype and detection of glycated hemoglobin (HbA1c) by mass spectrometry. Both methods are very robust, specific, fast and easy to handle. Only two microliters of human serum or hemolysate is needed.