Enrichment of Phosphorylated Peptides
Protein phosphorylation influence many biochemical processes in cells. Signal transduction and regulation of protein functionality mediated by phosphorylation are the most studied situations. Many analytical methods have been developed to monitor the status of protein phosphorylation, because its dysfunction is often related with a wide range of diseases and is so a challenging task for many researchers.
AffiPro offers alternative to conventional approaches for detection of phosphorylated peptides. The patented technology developed for functionalizing MALDI targets enables robust, fast and cheaper analysis of phosphorylated peptides by mass spectrometry. Single protein or very complex proteins mixture such as cell lysate are applied on the TiO2 modified surface just by pippeting or by using automated fraction collectors.
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